I can’t imagine It’s over 9000 ever dying.
I cast magic missile at the darkness.
Whenever someone turns to look behind them, I turn them into a pillar of salt.
It’s just this fun thing I do.
Mr T Ate My Balls, a member of the Ate My Balls Webring
kids today don’t know what a webring even is
C-C-C-COMBO BREAKER!!!
I could not find a cave art meme, so I will go with this one.

These fine owls are still living rent free in my head.
But the worst are probably Goatse, 2girls1cup, 1guy1jar, all the nazi ascii stuff. Modern day memers have it easy.

I don’t use any memes.

kilroy

the oldest for me is the lotr one about the gif: “the meme can not be destroyed by any power we have here”. i still got this via cd, because Internet wasn’t so much of a thing yet.

I think I first saw this on 4Chan circa like 2005? It lives rent free in my head.

Forsooth! This tapestry hath been altered. My knowledge doth derive from the stitching, as well as having observ’d many such forgeries in mine time.
The Bayeux Tapestry is probably one of the oldest memes (or at least the base for a set of memes) still in use in the digital age. So many earlier others based on wordplay or written text in general are probably incomprehensible now except to specific Tolkienesque ancient language nerds, since modern languages have mutated so much since medieval times, and are therefore lost.
Second place may belong to the Cool S.